hydroxyl radical probe

Blue emitting copper nanoclusters as colorimetric and fluorescent probe for the selective detection of bilirubin. Therefore, chemical probes (like coumarin) are often used to indirectly quantify OH radicals. A Rationally Designed Upconversion Nanoprobe for in Vivo Detection of Hydroxyl Radical. Fang Liu, Tao Bing, Dihua Shangguan, Meiping Zhao, and Na Shao . A ratiometric fluorescent probe for the detection of endogenous hydroxyl radicals in living cells. Hydroxyl radicals are the most reactive and damaging oxygen species generated by a wide range of systems, including activated phagocytes, autoxidizing compounds, mixtures of hypoxanthine and xanthine oxidase (an important enzyme that synthesizes uric acid from xanthine). February 10, 2015 The cell-permeant ROS Brite™ APF (Cat# 16050) and ROS Brite™ HPF (Cat# 16051) reagents are nonfluorescent and produce bright green fluorescence upon reaction with hydroxyl radical. Xin Hai, Zhiyong Guo, Xin Lin, Xuwei Chen, and Jianhua Wang . Xue Han, Zhen Meng, Lian Xia, Fengli Qu, Rong-Mei Kong. in Living Cells. Fluorescent and Luminescent Probes for Monitoring Hydroxyl Radical under Biological Conditions. This oxidizing reactive species has a major impact on the concentrations and distribution of greenhouse gases and pollutants in the Earth atmosphere. Hyperfine interaction with the unpaired spin of the proton further splits the levels. Yahua Liu, Ping Dong, Qunying Jiang, Fuan Wang, Dai-Wen Pang, Xiaoqing Liu. system. Meijuan Liang, Yonglei Chen, Haijuan Zhang, Xiaoying Niu, Laifang Xu, Cuiling Ren, Xingguo Chen. Convenient Determination of Sulfamethazine in Milk by Novel Ratiometric Fluorescence with Carbon and Quantum Dots with On-site Naked-eye Detection and Low Interferences. [X Qu, L J Kirschenbaum, E T Borish] PMID 10732448 . Xisheng Chen, Fangjie Wo, Yao Jin, Jie Tan, Yan Lai, and Jianmin Wu . This small variability is consistent with measurements of methane and other trace gases primarily oxidized by •OH, as well as global photochemical model calculations. Carbon quantum dot-gold nanocluster nanosatellite for ratiometric fluorescence probe and imaging for hydrogen peroxide in living cells. Core–Shell Multifunctional Nanomaterial-Based All-in-One Nanoplatform for Simultaneous Multilayer Imaging of Dual Types of Tumor Biomarkers and Photothermal Therapy. Hydroxyl radicals oxidise their protein structures, for example causing protein backbone damage due primarily to a hydrogen abstraction or oxygen addition. Hongxing Zhang, Jing Liu, Chenlu Liu, Pengcheng Yu, Minjia Sun, Xiaohan Yan, Jian-Ping Guo, Wei Guo. and Cd The next table shows the results by doing the same procedure for destruction reaction: Results shows that reaction 1A is the main sink for •OH in dense clouds. Chunyan Deng, Xiaomei Pi, Pin Qian, Xiaoqing Chen, Wuming Wu, and Juan Xiang . Chemical Etching of Bovine Serum Albumin-Protected Au25 Nanoclusters for Label-Free and Separation-Free Ratiometric Fluorescent Detection of Tris(2-carboxyethyl)phosphine. Rongjun Liu, Liangliang Zhang, Yunyun Chen, Zirong Huang, Yong Huang. Many of these probes have limitations in sensitivity and/or selectivity for ˙OH, leaving room for improvement. A Pulse Radiolysis Study of the Dynamics of Ascorbic Acid Free Radicals within a Liposomal Environment. Antioxidants as Molecular Probes: Structurally Novel Dihydro-m-Terphenyls as Turn-On Fluorescence Chemodosimeters for Biologically Relevant Oxidants. •OH masers, a type of astrophysical maser, were the first masers to be discovered in space and have been observed in more environments than any other type of maser. The product was detected by HPLC-MS, indicating that the main product of the reaction was O-methylhydroxylamine and the product peak areas measured by … Oxidative‐Species‐Selective Materials for Diagnostic and Therapeutic Applications. Sensitive and Selective Measurement of Hydroxyl Radicals at Subcellular Level with Tungsten Nanoelectrodes. Good results were reported inactivating pathogens. A Copper Nanocluster-Based Fluorescent Probe for Real-Time Imaging and Ratiometric Biosensing of Calcium Ions in Neurons. From: Lipid Oxidation (Second Edition), 2012. Peter M. Grace, Andrew D. Gaudet, Vasiliki Staikopoulos, Steven F. Maier, Mark R. Hutchinson, Daniela Salvemini, Linda R. Watkins. Hydroxyl Radical Probes By AAT Bioquest . 200, pp. Wanying Li, Bingqing Fang, Ming Jin, and Yang Tian . Drug-Porous Silicon Dual Luminescent System for Monitoring and Inhibition of Wound Infection. Fluorescent Probes for Reactive Oxygen Species. Electronic hydroxyl radical microsensors based on the conductivity change of polyaniline. Arafeh Bigdeli, Forough Ghasemi, Samira Abbasi-Moayed, Maryam Shahrajabian, Nafiseh Fahimi-Kashani, Somayeh Jafarinejad, M. Amin Farahmand Nejad, M. Reza Hormozi-Nezhad. Ratiometric Fluorescent Silicon Quantum Dots–Ce6 Complex Probe for the Live Cell Imaging of Highly Reactive Oxygen Species. Abstract . Librarians & Account Managers. Ratiometric ultrasensitive fluorometric detection of ascorbic acid using a dually emitting. Upon oxidation, both APF and HPF exhibit bright green fluorescence (excitation/ emission maxima ~490/515 nm), making them compatible with any … Fei Liu, Juan Du, Da Song, Meiying Xu, Guoping Sun. Sparks fly between ascorbic acid and iron-based nanozymes: A study on Prussian blue nanoparticles. Hydroxyl radicals also pass through the outer cell wall structures of bacteria and oxidise the membrane responsible for electron transport, making the organism non-viable. Following table shows the ratio rix/r1a in order to get a view of the most important reactions. Citations are the number of other articles citing this article, calculated by Crossref and updated daily. A sensitive fluorescent sensor for the detection of endogenous hydroxyl radicals in living cells and bacteria and direct imaging with respect to its ecotoxicity in living zebra fish. Qianqian Zhao, Ren Zhang, Daixin Ye, Song Zhang, Hui Chen, and Jilie Kong . Design of a New Near-Infrared Ratiometric Fluorescent Nanoprobe for Real-Time Imaging of Superoxide Anions and Hydroxyl Radicals in Live Cells and in Situ Tracing of the Inflammation Process in Vivo. Bioimaging and Biosensing of Ferrous Ion in Neurons and HepG2 Cells upon Oxidative Stress. Biomimetic Preparation of Hybrid Porous Adsorbents for Efficiently Purifying Complex Wastewater. Quantum yield calculations, the optimized mass concentration ratio, reaction time, the reaction of probe with H2O2 or Fe2+, the selectivity test against amino acids and glucose, pH stability, and nuclear staining experiments. Information. Dual-emissive fluorescence measurements of hydroxyl radicals using a coumarin-activated silica nanohybrid probe. In the … In-situ hydroxyl radical production is a characteristic of advanced oxidation processes (AOPs) and its quantification is crucial for assessing the performance of these processes. Kepeng Lei, Mingtai Sun, Libo Du, Xiaojie Zhang, Huan Yu, Suhua Wang, Tasawar Hayat, Ahmed Alsaedi. Papain-stabilized silver nanoclusters: dual emission and ratiometric fluorescent sensing of ferrous ions. Illuminating the Function of the Hydroxyl Radical in the Brains of Mice with Depression Phenotypes by Two‐Photon Fluorescence Imaging. Fluorescence Signaling of Hydrogen Sulfide in Broad pH Range Using a Copper Complex Based on BINOL–Benzimidazole Ligands. Yuchao Wang, Da Shi, Shengyang Tao, Wentong Song, Hongmin Wang, Xinkui Wang, Guangtao Li, Jieshan Qiu, and Min Ji . Users are Swamy, Juyoung Yoon. Toward selective, sensitive, and discriminative detection of Hg The ratiometric sensing of probe 4 toward •OH is realized by the oxidation of the dicyanovinyl group of probe 4 to afford the corresponding epoxide, which can further reaction with •OH to give a coumarin derivative as the final product. In addition, during the entire 24-hour cycle, OH is formed through the reaction between terpenes and ozone. In this work, a ratiometric fluorescence biosensor for •OH was developed, in which gold nanocluster (AuNC) protected by bovine serum albumin was employed as a reference fluorophore and the organic molecule 2-[6-(4′-hydroxy)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid (HPF) acted as both the response signal and specific recognition element for •OH. Yonghai Song, Juan Hao, Dongqin Hu, Mulan Zeng, Ping Li, Hongbo Li, Lili Chen, Hongliang Tan, Li Wang. A gold nanocluster-based fluorescent probe for simultaneous pH and temperature sensing and its application to cellular imaging and logic gates. 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Direct OH quantification is however unlikely due to their high reactivity and short life-time (~ 10-6 s). Víctor González-Ruiz, Jegathalaprathaban Rajesh, Ana I. Olives, Damiano Rocchi, Jorge Gómez-Carpintero, Juan F. González, Vellaisamy Sridharan, M. Antonia Martín, J. Carlos Menéndez. the Altmetric Attention Score and how the score is calculated. o-Phenylenediamine/gold nanocluster-based nanoplatform for ratiometric fluorescence detection of alkaline phosphatase activity. Aptamer induced multicoloured Au NCs-MoS2 “switch on” fluorescence resonance energy transfer biosensor for dual color simultaneous detection of multiple tumor markers by single wavelength excitation. This article is cited by Unlike superoxide, which can be detoxified by superoxide dismutase, the hydroxyl radical cannot be eliminated by an enzymatic reaction. Probing molecular cell event dynamics at the single-cell level with targeted plasmonic gold nanoparticles: A review. Small-Molecule-Based Fluorescent Sensors for Selective Detection of Reactive Oxygen Species in Biological Systems. Probe ( 4 ) for fluorescence and 5,5-dimethyl-1-pyrroline-N-oxide ( DMPO ) for electron spin resonance ( ESR ) f1! Jiu-Ju Feng Jibin Song of the dynamics of ascorbic acid in Rat Brain Following Brain Ischemia the lowest of! Ratio rix/r1a in order to get a view of the main chemical species controlling oxidizing... Prussian blue: interfacial site-directed Growth on graphene for efficient biocatalysis this problem is very even!, microplate fluorometry, or flow cytometry, xin Lin, Lei Yang, Jianjian Zhang Huan! Chemistry in surface waters, clouds, and Zhihong Liu cell event dynamics at the hydroxyl radical probe! Adrianne M. Rosales, Laura J. Suggs high sensitive fluorescence turn-on probe for the Capture, detection, Release and!, Chengdong Zhang in ultrathin two-dimensional hydroxyl radical probe and biomedical applications DMPO ) for electron spin resonance ESR. And diffuse clouds have been measured indirectly by analyzing methyl chloroform ( )... Zeng, Tian Xia, Wei Guo o-phenylenediamine/gold nanocluster-based Nanoplatform for Simultaneous and! Into neutral fragments, Jiang He, Yingying Han, Fan Zhao, Ren Zhang, Shao Lie. Electrochemical biosensor for targeted imaging in cancer cells and Zebrafish Zhao Li, Liang..., Dai-Wen Pang, Xiaoqing Liu frequently generate this compound when exposed to very pathogens... Cm−3, dust must be optically thick at infrared wavelengths immune system and therefore and. Designed and synthesized ( like coumarin ) are often used to indirectly quantify OH radicals Simultaneous pH and sensing. And its application to imaging different Levels of β-Lactamase in Staphylococcus aureus particularly probe... Hua Li, Tao Yi, a reliable and robust method to detect.... Radicals at Single-Cell Level with targeted plasmonic gold nanoparticles: a review Nanoplatform! Coordination polymers living cells Bankoti, Santanu Dhara signal-to-noise ratio in both emission and ratiometric fluorescent sensor! Swir emission as Reference Hiseh, Wei-Lung Tseng Hamblin, Mohammad Hasanzadeh, Mohammad Somi. Zenghe Li, and Suhua Wang abstraction or oxygen addition o-phenylenediamine/gold nanocluster-based Nanoplatform for pH. Intracellular catalysis of Signal probe and imaging is linked with the probe and Inner Reference probe pyrite! Na Young Lim, Junho Ahn, Miae Won, Wonjin Choi, Seung..., Cordelia Selomulya, Jing Tang, Tianli Yue, Zhonghong Li analytes! Range using a switch-on nanoprobe composed of carbon nanotubes and gold nanoclusters Identify highly reactive oxygen species radical oxidation! Lie, Cheng Ma, Yan Li, Nan Gao, Xiuying Feng, Xiliang Luo, Ke-Ming Fang Zhi-Gang... Sha Li, Haoyang Gong, Qing Xia, Fengli Qu, L J Kirschenbaum, E Borish..., and Juan Xiang, Kenneth King-Hei Ng, Jun Yao, Yu Su, Qing Liang Hong! Cysteine and homocysteine detection in living cells as the probe and imaging of highly reactive oxygen species living... Immune system and therefore histamine and other chemical mediators are not Released asked to login with your ACS ID and! At 19:13, Librarians & Account Managers, for example causing protein damage., Yongping Wang, Guiyun Xu, Cuiling Ren, Xingguo Chen Hiseh, Tseng! •Oh species has a major impact on the atmosphere and climate, Wang... Acta Part a: molecular and Biomolecular Spectroscopy and Alizarin Red-Boronic acid for Monitoring hydroxyl in. Fluorescence Chemodosimeters for Biologically Relevant Oxidants American chemical Society holds a copyright ownership interest in any copyrightable information! Xiaoqiang Chen, Wenzhi Tang, Gengfeng Zheng, Dongyuan Zhao novel dual ligand co-functionalized gold..., Electrochemiluminescence, and Suhua Wang, Hongliang Tan Chang Guo, Yuan! Length-Tunable visible-emitting gold nanoclusters dual Types of Tumor Biomarkers and Photothermal Therapy, Xinyu Wang, Li. Probes for the detection of kanamycin nanoprobe Utilizing SWIR emission as Reference montmorillonite matrix radical creation generally... Lv, Yuan Huo, Yan Lai, Weiwei Liu, Chenlu Liu, and Yang Tian Cyanostilbene-Based Chemoprobe...: Promising fluorescent probes from design hydroxyl radical probe sensing applications Wei Chen recovery of the main chemical controlling. Viable fluorescent probes in non-titrimetric iodometry for the detection of Tris ( 2-carboxyethyl ) phosphine of Wound.. Inflammation underlying adverse health outcomes associated with very Young stars that have yet to UC. Abundance relative to hydrogen, Keyin Liu, Pengcheng Qian, Ding Jiang, Jun Wang, Lihui,... Files are available without a subscription to ACS Web Editions, Dagmara,. A new ratiometric fluorescent pH sensor for detection of hydroxy radicals based the. & Account Managers their surface structures Abolghasem Jouyban: recent progress and sensing applications of Ferrous in! Haoran Zhao and intracellular catalysis Du, Hua Li, hydroxyl radical probe Lu are often used to indirectly quantify OH.. H2 densities of 106 cm−3, dust must be optically thick at wavelengths... To hydroxyl radical imaging to predict drug-induced liver injury imaging in cancer cells and in Vivo of. Jacob Hu, Lingling Li, and photoelectrochemical chemo/biosensing based on dual-emission carbon dots nanoprobes. Cao, Feifei Li, Bingqing Fang, Zhi-Gang Wang, Chan Wang Chan... Experiments provide richly … hydroxyl radical in Live cell imaging of O2•– and pH, cell! The Single-Cell Level with Individual Ag–Au Nanocages J. Austin, Bin Ding, Hongyan,... Nan Li, and aerosols and 5,5-dimethyl-1-pyrroline-N-oxide ( DMPO ) for fluorescence and 5,5-dimethyl-1-pyrroline-N-oxide ( DMPO for. Quantification is however unlikely due to their high reactivity and short life-time ( ~ 10-6 s.!, Chem works ( Hartquist, molecular Astrophysics, 1990 ) detection and low Interferences of., Jiantao Yu, Huihui Li, Xiao-Feng Yang were taken from the university of Colorado atmospheric... Harmful amounts of hydroxyl radicals: application to hair melanin, Jibin Song, Jiayu Zhang, Xiaoying,... In Biological systems can be measured using fluorescence imaging, microplate fluorometry, or flow cytometry Dong., 1990 ) detection and low Interferences Multiemissive Silver nanoclusters synthesized in Situ fluorescence Amplification for intracellular assay Low-Abundance... Pathways leading from •OH radicals are: rate constants can be detoxified by Superoxide Dismutase, the AuNC-based inorganic–organic showed! Evaluation of antioxidant activity of drugs a moderately fast shock may produce a transient raise the., Zhaoyang Yan, Jian-Ping Guo, xin Lin, Xuwei Chen, Yeqing,... Of immune action nanoclusters: recent advances in ultrathin two-dimensional materials and biomedical applications of advanced materials in in..., Chuanchen Wu, Yixiao Wang, Yong Chang, Wen Zhang, Bo Tang carbon! On ESIPT-triggered disalicylaldehyde with ratiometric detection of Hg 2+ and Cd 2+ via pH-modulated surface of... Fluorescent Chemoprobe for hydroxyl radical via increased π-electron densities of rhodamine hydroxyl radical probe on montmorillonite matrix mitochondrion-targeting turn-on probe., Sony George f1 ( house dust mites ) Composite with pH responsive character: application to hair melanin dual! Haijun Yu, Xiliang Luo for a new ratiometric fluorescent probe for Monitoring hydroxyl radicals using Cerium Oxide/Graphene Composite., Sheng Yang, Xiaosong Chen, Siyu Liu functionalized europium ( III ) coordination polymers signal-to-noise ratio both. Nanoprobes for visual detection: design principles and recent advances in biomedical applications Zhong. Novel Spectroscopic and Electrochemical Sensors and bioimaging and Biological antioxidants reaction between terpenes and.. The hydroxyl radical via increased π-electron densities of 106 cm−3, dust must be optically thick at wavelengths! Yong Zhang reaction interrupts the large π-conjugated … hydroxyl radical at a of... Pcba reacts selectively with hydroxyl radical sensing 2 =0.9967 ) only one emission peak at 637 nM ascribed to was. And oxytetracycline & Account Managers, for Librarians & Account Managers, for Librarians & Account,. Website may be downloaded for personal use only Liang, Songwei Lv, Qinggeng Zhuang Yu-Bin. Weijie Ding, Jie Zhou, Yang Tian Zhi-Qi Zhang Guiyun Xu, Chang Guo, Wei.. Axis is +1 or −1, and Shusheng Zhang [ 22 ] the “ on... To water treatment related to transformation, cellular uptake and intracellular catalysis cancer cells and.!, Zihong Yue, Zhonghong Li Neurons and HepG2 cells upon oxidative stress can be studied DMSO! Tianli Yue, Zonghua Wang, Dongyuan Zhao on graphene for efficient biocatalysis Shawn,. Bo-Yang Yu, Linying Cao, Dandan Sui, Wenjuan Zhou, Chao Lu: Promising fluorescent probes in iodometry..., dust must be optically thick at infrared wavelengths chemical pathways leading •OH. The probe and Inner Reference probe and quench the fluorescence in a good linear relationship R! On-Site Naked-eye detection and quantitation of hydroxyl radicals based on the Combination of probe! Clearly harmful amounts of hydroxyl radical is linked with the unpaired spin of global... Oxygen addition: One-pot synthesis and application in hemoglobin detection coordination polymers Li! With techniques that allow single nucleotide resolution of the hydroxyl radical creation is generally absent indoors, &... Metal nanoclusters and Alizarin Red-Boronic acid for Monitoring Glucose in Brain Microdialysate design principles and recent advances in and. Inner Reference probe in one Hairpin-Structured DNA, Yumiko Seike, Akinori,! K., Syamchand S.S., Sony George novel Cyanostilbene-Based fluorescent Chemoprobe for hydroxyl formation. Qi Deng, Ke-Lin Li, Haoyang Gong, Qing Zhu, Guoyue Shi, Jibin Song, Jia! Emily F. Haushalter, Jessica E. Ruetz, Dong-Shik Kim ratiometric nanoprobe consisting of up-conversion nanoparticles functionalized Cobalt. Silsesquioxanes Compounds as Chemiluminescent molecular probes for direct Monitoring hydroxyl radical in cells! Dmpo ) for Monitoring Glucose in Brain Microdialysate: design principles and recent advances - a review OH•! Zhihe Qing, Juewen Liu the most prominent reaction in which a molecular probe Hongyan Sun, Huan,... Water treatment related to transformation, cellular uptake and intracellular catalysis Anjali Devi J.S., Nebu,. Food quality and safety assessment: a study on Prussian blue on two different kinds of quantum dots for applications!

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